This newest volume in the groundbreaking new series, “Bioprocessing in Food Science,” focuses on the latest processes, industrial applications, and leading research on microbes in the food industry, for engineers, scientists, students, and other industry professionals.
Table of ContentsPreface
1. Food Microbiology: Fundamentals and TechniquesRaina Jain, Prashant Bagade, Kalpana PatilDoke and Ganesh Ramamurthi
1.1 Introduction
1.2 Food Microbiology: A Historical Perspective
1.3 Beneficial Microbes in Food
1.3.1 Factors Influencing Microbial Growth in Food
1.3.2 Food Fermentation
1.3.3 Probiotics
1.3.4 Microbial Bio-Preservatives
1.4 Harmful Microbes in Food
1.4.1 Factors Influencing Food Spoilage
1.4.2 Indicators of Food Spoilage
1.4.3 Foodborne Infections and Intoxications
1.4.4 Food Preservation to Control Spoilage
1.5 Classical Food Microbiological Techniques
1.6 Advances in Food Microbiological Techniques
1.6.1 Protein-Based Methods
1.6.2 DNA-Based Methods
1.6.3 Biosensor-Based Methods
1.6.4 AI and ML Applications
1.7 Regulations Governing Food Microbiology
1.8 Conclusions
References
2. Fermented Foods in Health and Disease PreventionMonalisa Sahoo, Pramod Aradwad, Nikita Sanwal, Jatindra Kumar Sahu, Vivek Kumar and S. N. Naik
2.1 Fermentation
2.1.1 Types of Fermentation
2.1.2 Bioactive Compounds
2.2 Traditional Fermented Food
2.3 Application of Fermentation to Food
2.3.1 Fruits and Vegetables-Based Fermented Products
2.3.1.1 Kimchi
2.3.1.2 Sauerkraut
2.3.1.3 Boza
2.3.1.4 Wine
2.3.1.5 Vinegar
2.3.2 Fermented Products from Roots and Tubers
2.3.3 Cereals and Legumes-Based Fermented Products
2.3.3.1 Beer
2.3.3.2 Sourdough
2.3.4 Fermentation of Meat, Fish and Seafood
2.3.4.1 Meat Sausages
2.3.5 Fermented Dairy Products
2.3.5.1 Kefir
2.3.5.2 Koumiss
2.3.5.3 Cheese
2.3.5.4 Yogurt
2.3.6 Other Fermented Products
2.4 Effects of Fermentation on Nutrients
2.4.1 Carbohydrates
2.4.2 Proteins
2.4.3 Fat
2.4.4 Micronutrients
2.4.4.1 Vitamins
2.4.4.2 Minerals
2.4.5 Phytonutrients
2.5 Health Benefits of Fermented Foods and Beverages
2.5.1 Antiallergic Effect
2.5.2 Antioxidant Activity
2.5.3 Antihypertensive Activity
2.5.4 Antimicrobial Activity
2.5.5 Alleviating Cholesterol Levels
2.5.6 Anticancer Activity
2.5.7 Effect on Gastrointestinal Diseases
2.5.8 Antidiabetic Effect
2.6 Food Safety and Quality Control
2.7 Conclusions and Future Perspectives
References
3. Probiotic Dairy FoodsGökçe Eminoglu, H. Ceren Akal and H. Barbaros Ozer
3.1 Introduction
3.2 Classification and Phylogenetic Properties of Probiotic Microorganisms
3.2.1 Lactobacillus Genus
3.2.2 Bifidobacterium Genus
3.2.3 Lactococcus Genus
3.2.4 Streptococcus Genus
3.2.5 Enterococcus Genus
3.3 Probiotics in the Dairy Matrix
3.4 Probiotic Dairy Products
3.4.1 Probiotic Yogurt
3.4.2 Probiotic Cheese
3.4.3 Probiotic Ice Cream
3.4.4 Probiotic Dairy-Based Beverages
References
4. Dairy Probiotic ProductsCallebe Camelo Silva, Silvani Verruck, Marco Di Luccio, Tatiana C. Pimentel, Marcia Cristina Silva, Erick Almeida Esmerino and Adriano Gomes da Cruz
4.1 Introduction
4.2 Fermented Milks
4.2.1 Yogurt
4.2.1.1 Effect of Probiotic Supplementation on Yogurt Properties
4.2.1.2 Potential Human Health Benefits of Supplementing Yogurts with Probiotics
4.2.2 Kefir
4.2.3 Cheese
4.2.4 Butter
4.2.5 Ice Cream
4.2.6 Dairy Desserts
4.3 Conclusions and Perspectives
References
5. Design Schematics, Operational Characteristics and Process Applications of BioreactorsVishwajeet Gaikwad, Anil Panghal, Shubham Jadhav, Sunil Kundu, Namita Singh and Navnidhi Chhikara
5.1 Introduction
5.2 Fermenter Design and Operations
5.2.1 Structural Material
5.2.2 Features of Fermenter Design
5.3 Fermenter Configuration
5.3.1 Batch Process
5.3.1.1 Batch Fermenter Design
5.3.2 Continuous Process
5.3.2.1 Continuous Stirred Tank Reactor (CSTR)
5.3.2.2 Plug Flow Reactor (PFR)
5.3.3 Fed Batch or Semi-Continuous Process
5.3.3.1 The Constant Volume or Fixed Volume Fed-Batch Reactors
5.3.3.2 The Variable Volume Fed-Batch Reactors
5.4 Types of Fermenter
5.4.1 Stirred Tank Fermenter
5.4.2 Airlift Fermenter
5.4.3 Bubble Column Reactor
5.4.4 Fluidized Bed Reactor
5.4.5 Packed Bed Reactor
5.4.6 Membrane Bioreactor
5.5 Factors Influencing Operation of Fermenters
5.5.1 Heat Transfer
5.5.2 Mass Transfer
5.5.3 Scale-Up in Fermenter
5.6 Conclusion
References
6. Enzymes in Food Industry and Their Regulatory OversightMegha Dhingra and Jasvir Singh
6.1 Introduction
6.2 Production of Enzymes
6.2.1 Sources of Enzymes
6.2.2 Strain Selection
6.2.3 Fermentation
6.2.4 Pretreatment
6.2.5 Downstream Processing
6.2.6 Enzyme Formulation
6.2.6.1 Formulation of Solid Products
6.2.6.2 Formulation of Liquid Products
6.3 Applications of Enzymes in Food Industry
6.3.1 Enzymes in Starch Processing
6.3.2 Enzymes in Baking Industry
6.3.3 Enzymes in Brewing Industry
6.3.4 Enzymes in Dairy Industry
6.3.5 Enzymes in Fruits and Vegetables Processing Industry
6.3.6 Other Applications
6.4 Safety Evaluation of Enzymes
6.4.1 Global Frameworks for Safety Evaluation
6.4.2 Environmental Health Criteria 240: Principles and Methods for the Risk Assessment of Chemicals in Food
6.4.3 JECFA Guidelines on Safety Assessment of Enzymes
6.5 Global Regulatory Frameworks
6.6 Regulatory Framework in India
References
7. Functional and Nutraceutical Potential of Fruits and VegetablesSamandeep Kaur, Umexi Rani and Parmjit Singh Panesar
7.1 Introduction
7.2 Biochemistry of Fruits and Vegetables
7.2.1 Polyphenols and Flavonoids
7.2.2 Carotenoids
7.2.3 Vitamins
7.2.4 Glucosinolates
7.2.5 Phytoestrogens
7.3 Nutritional Composition of Fruits and Vegetable By-Products
7.4 Extraction of Bioactives from Fruits and Vegetables
7.4.1 Conventional Extraction Techniques
7.4.2 Novel Extraction Techniques
7.5 Processing Methods Used for Development of Functional Foods from Fruits and Vegetables
7.5.1 Fermentation
7.5.1.1 Fermented Products
7.5.2 Food Fortification
7.5.2.1 Superfoods
7.5.3 Encapsulation
7.6 Fruits and Vegetable-Based Nutraceuticals
7.7 Influence of Processing Methods on Functional Ingredients
7.8 Influence of Storage on Functional Ingredients
7.9 Future of Functional Foods
Conclusion
References
8. Microbes as Bio-Factories for the Valorization of Fruit and Vegetable Processing WastesShivali Banerjee and Amit Arora
8.1 Introduction
8.2 Microbial Bio-Processing of Fruit and Vegetable Wastes
8.3 Valuable Commodities from Fruit and Vegetable Waste
8.3.1 Fermented Beverages
8.3.2 Food Colorants
8.3.3 Single-Cell Protein (SCP)
8.3.4 Flavor and Fragrance
8.3.5 Enzymes
8.3.6 Organic Acids
8.3.7 Biopesticides
8.3.8 Plant Growth Regulators
8.3.9 Polysaccharides
8.3.10 Biofuels
8.4 Technical Challenges, Economics and Future Prospective
8.5 Conclusion
References
9. Solid-State FermentationManish Tiwari, Rashmin Dhingani, Nandani Goyal, Bhavesh Joshi and R.V. Prasad
9.1 Introduction
9.1.1 Principle of SSF
9.1.2 Process of Solid-State Fermentation
9.2 History of Solid-State Fermentation (SSF)
9.3 Factors Affecting SSF
9.3.1 Inoculum Type
9.3.2 Moisture and Water Activity
9.3.3 pH
9.3.4 Temperature
9.3.5 Substrates
9.3.6 Aeration and Agitation
9.3.7 Nutritional Factors
9.4 Types of Solid-State Fermentation
9.4.1 Aerobic SSF
9.4.2 Anaerobic SSF
9.5 Application of SSF Carried Out on Inert Support Materials
9.5.1 Enzyme
9.5.2 Organic Acids
9.5.3 Bacteriocins
9.5.4 Flavour and Aroma Compounds
9.6 Modern Aspects of Solid-State Fermentation
9.6.1 Advancement in the Design of Bioreactors for Fermentation
9.6.2 Bioreactors in Solid-State Fermentation
9.6.2.1 Tray Bioreactor
9.6.2.2 Packed-Bed Bioreactor
9.6.2.3 Intermittent or Continuously Mixed SSF Bioreactor
9.6.2.4 Rotating Drum Bioreactors (RBD)
9.6.2.5 Modular Bioreactor
9.6.2.6 Novozymes Bio A/G Bioreactor
9.6.2.7 Air-Solid Fluidized Bed Bioreactor
9.6.3 Biotechnology in SSF
9.7 Challenges to SSF
9.8 Conclusions
References
10. Pigments Produced by Fungi and Bacteria from Extreme EnvironmentsGraciéle Cunha Alves de Menezes, Tiago Daniel Madureira de Medeiros, Igor Gomes de Oliveira Lima, Maurício Bernardo da Silva, Aline Cavalcanti de Queiroz,
Alysson Wagner Fernandes Duarte, Valéria Maia de Oliveira, Luiz Henrique Rosa and Juliano Lemos Bicas
10.1 Introduction
10.2 Extreme Environments
10.2.1 Polar Ecosystems
10.2.2 Alpine and High-Altitude Ecosystems
10.2.3 Desert Ecosystems
10.2.4 Saline Ecosystems
10.2.5 Volcanic Ecosystems
10.3 Extremophilic Microorganisms
10.3.1 Fungi
10.3.2 Bacteria
Conclusion
Acknowledgments
References
11. Commercially Available Databases in Food MicrobiologyPriyanka Rohilla, Anju Kumari, Sapna Birania and Monika
11.1 Introduction
11.2 Functions of a Databases
11.3 Need for Databases
11.4 Predictive Microbiology in Foods
11.4.1 History of Predictive Microbiology
11.4.2 Terminology in Predictive Microbiology
11.5 Predictive Microbiology and Its Models
11.5.1 Experimental Design
11.5.2 Data Generation
11.6 Rapid Methods of Data Generation
11.6.1 Turbidimetry
11.6.2 Flow Cytometry
11.6.3 Microscopy and Image Analysis
11.6.4 Electrochemical Methods: Impedance and Conductance
11.7 Predictive Models
11.7.1 Primary Models
11.7.2 Growth Models
11.7.2.1 Mechanistic Functions
11.7.2.2 Logistic and Linear Functions
11.7.2.3 Buchanan Three-Phase Linear Model
11.7.2.4 Compartmental Models
11.7.3 Inactivation Models
11.7.3.1 Bigelow Model (Linear Model)
11.7.3.2 Weibull Model
11.7.3.3 Shoulder/Tail Models
11.7.4 Secondary Models
11.7.4.1 Polynomial Models
11.7.4.2 Square Root Models
11.7.4.3 The Gamma Concept and the Cardinal Parameter Model (CPM)
11.7.4.4 Artificial Neural Networks (ANNs)
11.7.5 Secondary Inactivation Models
11.7.5.1 Probabilistic Modeling
11.8 Guidelines for Modeling the Shelf Life of Foods
11.9 Databases in Foods
11.9.1 ComBase (Combined Database on Predictive Microbiology Information)
11.9.2 Microbial Responses Viewer (MRV): A New ComBase-Derived Database
11.9.3 Microbionet: A Database for the Visualization and Exploration of Food Bacterial Communities
11.9.4 LabBase: An Innovative Food Microbial Growth Responses Database
11.9.5 “MicroHibro”: A Software Tool for Predictive Microbiology and Microbial Risk Assessment in Foods
11.10 QMRA (Quantitative Microbial Risk Assessment)
11.10.1 Seafood Spoilage Predictor
11.11 Other Databases
11.11.1 Sym’previus
11.12 Future Prospects
References
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